Who invested in TwoStep Therapeutics?
TwoStep Therapeutics's investors include NFX, 2048 Ventures, Insight Partners and Alexandria Venture Investments.

United States · Series A startup
Funding summaryUpdated 26 Oct 2026
$84Mraised across 2 rounds since 2026, from 2 sources
Each circle is a round, placed by date and sized by amount. The line above is the running total.
Source recordedSEC filing: $21M sold, first sale Jan 2026
From TwoStep Therapeutics's own website: 1 on the team.
The busiest backers across the latest rounds. A filled square is a round they joined; a dark one, a round they led.
TwoStep Therapeutics's investors include NFX, 2048 Ventures, Insight Partners and Alexandria Venture Investments.
TwoStep Therapeutics has raised $84M across 2 rounds since 2026.
TwoStep Therapeutics's most recent round was a Series A of $63M in Sept 2026.
Its most recent round, $63M in Sept 2026, is also its largest on record.
TwoStep Therapeutics's first round on record, in Jan 2026, was backed by NFX, Alexandria Venture Investments and 2048 Ventures.
TwoStep Therapeutics's latest named round is a Series A, in Sept 2026.
TwoStep Therapeutics is based in the United States.
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