# US Department of Energy's AI Investments: 13 Startups in Its Portfolio (2026) > The 13 AI startups US Department of Energy has backed, by stage: $457M raised between them. From the rounds we track; updated nightly. Source: https://millionrounds.com/portfolio-maps/u-s-department-of-energy-ai-portfolio (Millionrounds; every figure on the page links to its filing or article) ## Stage not reported 9 - [eXoZymes](https://millionrounds.com/company/exozymes) - [Equiwatt](https://millionrounds.com/company/equiwatt) - [EnviTrace](https://millionrounds.com/company/envitrace) - [Bastazo](https://millionrounds.com/company/bastazo) - [Wildlife Imaging Systems](https://millionrounds.com/company/wildlife-imaging-systems) - [FR8relay](https://millionrounds.com/company/fr8relay) - [Mizar Imaging](https://millionrounds.com/company/mizar-imaging) - [Zeus AI](https://millionrounds.com/company/zeus-ai) - [RadiaSoft](https://millionrounds.com/company/radiasoft) ## Pre-seed and seed 4 - [Mosaic](https://millionrounds.com/company/mosaic) - [*E* Eden](https://millionrounds.com/company/eden) - [Magnefy](https://millionrounds.com/company/magnefy) - [Iris Light Technologies](https://millionrounds.com/company/iris-light-technologies) ## Segment by segment ### Stage not reported 9 startups · $11M raised - [**eXoZymes**](https://millionrounds.com/company/exozymes) eXoZymes develops enzyme-based biomanufacturing solutions that use AI-enhanced, cell-free enzyme systems. United States $5.0M - [**Equiwatt**](https://millionrounds.com/company/equiwatt) Equiwatt is a digital platform enabling households to get paid for saving energy at peak times. United Kingdom $2.1M - [**EnviTrace**](https://millionrounds.com/company/envitrace) EnviTrace is a creation of cutting-edge machine learning and artificial intelligence (AI/ML) techniques and technologies. United States $1.1M - [**Bastazo**](https://millionrounds.com/company/bastazo) Bastazo is an AI-driven cybersecurity platform focused on operational technology (OT) security and vulnerability remediation for c United States $1.1M - [**Wildlife Imaging Systems**](https://millionrounds.com/company/wildlife-imaging-systems) Wildlife Imaging Systems develops a machine vision platform to help the research community conserve wildlife through video capture. United States $1.1M - [**FR8relay**](https://millionrounds.com/company/fr8relay) FR8relay is transforming supply-chain and logistics using relay and AI United States $300K - [**Mizar Imaging**](https://millionrounds.com/company/mizar-imaging) Mizar Imaging is a light sheet illumination system that is modular and designed for high-resolution imaging. United States $257K - [**Zeus AI**](https://millionrounds.com/company/zeus-ai) Zeus AI Ltd is a technology company based in London that specializes in AI agents for sales engagement and customer conversation automation. United States $250K ### Pre-seed and seed 4 startups · $446M raised - [**Mosaic**](https://millionrounds.com/company/mosaic) AI Workforce Management Software United States $341M - [*E* **Eden**](https://millionrounds.com/company/eden) United States $104M - [**Magnefy**](https://millionrounds.com/company/magnefy) AIoT Predictive Monitoring for Power Infrastructure United States $1.4M - [**Iris Light Technologies**](https://millionrounds.com/company/iris-light-technologies) Iris Light Technologies is a Chicago-based company specializing in nanotechnology and silicon photonics. United States $120K **How this map is made.** Every startup here works in AI and has raised money in a round we track that names US Department of Energy as an investor. Each sits under the stage of its latest round. Rebuilt nightly; updated 15 November 2026. ## More portfolio maps [All portfolios](https://millionrounds.com/portfolio-maps) [Sector portfolio **US Department of Energy's Energy portfolio** 69 startups · $14B](https://millionrounds.com/portfolio-maps/u-s-department-of-energy-energy-portfolio)[Sector portfolio **US Department of Energy's Cleantech portfolio** 43 startups · $3.0B](https://millionrounds.com/portfolio-maps/u-s-department-of-energy-cleantech-portfolio)[Sector portfolio **US Department of Energy's Chemicals & Materials portfolio** 32 startups · $1.0B](https://millionrounds.com/portfolio-maps/u-s-department-of-energy-chemicals-materials-portfolio)[Sector portfolio **US Department of Energy's Manufacturing portfolio** 17 startups · $2.6B](https://millionrounds.com/portfolio-maps/u-s-department-of-energy-manufacturing-portfolio)[Sector portfolio **US Department of Energy's Big Data & Analytics portfolio** 16 startups · $19M](https://millionrounds.com/portfolio-maps/u-s-department-of-energy-big-data-analytics-portfolio)[Sector portfolio **US Department of Energy's Biotech portfolio** 13 startups · $109M](https://millionrounds.com/portfolio-maps/u-s-department-of-energy-biotech-portfolio) ## Raising from investors like US Department of Energy? 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