# TwoStep Therapeutics funding: rounds and investors

> TwoStep Therapeutics has raised $84M across 2 rounds since 2026. Investors include NFX, 2048 Ventures and Insight Partners. Every round with its source.

Source: https://millionrounds.com/company/twostep-therapeutics (Millionrounds; every figure on the page links to its filing or article)

United States · [Series A startup](https://millionrounds.com/companies)

# Who invested in *TwoStep Therapeutics*

2 rounds we have observed, with the 4 investors named in them. Every figure came from a named source, and where the company reported a raise to the SEC itself, that is shown beside it.

Biotech

Raised 2 months ago

Funding summary Updated 26 Oct 2026

**$84M** raised across 2 rounds since 2026, from 2 sources

**$21M** Series A **$63M** Series A

- **Latest round:** Series A, $63M Sept 2026
- **Step-up i:** 3×
- **Investors:** 4

See who backed them

## The funding story

Each circle is a round, placed by date and sized by amount. The line above is the running total.

2026 2027

**$21M** Series A

**$63M** Series A

- **First round:** Series A, $21M
- **Biggest round:** $63M
- **Between rounds:** 8 months

## The rounds

- **Sept 2026** Series A **$63M** *1 new* *2 returning* [Insight Partners](https://millionrounds.com/fund/insight-venture-partners) [NFX](https://millionrounds.com/fund/nfx) [2048 Ventures](https://millionrounds.com/fund/2048-ventures) Source recorded
- **Jan 2026** Series A **$21M** *3 new* [NFX](https://millionrounds.com/fund/nfx) [Alexandria Venture Investments](https://millionrounds.com/fund/alexandria-venture-investments) [2048 Ventures](https://millionrounds.com/fund/2048-ventures) Source recorded SEC filing: $21M sold, first sale Jan 2026

## Founders and team

From TwoStep Therapeutics's own website: 1 on the team.

- **Joy Geallis** VP, Clinical

## Who was in which round

The busiest backers across the latest rounds. A filled square is a round they joined; a dark one, a round they led.

**Series A** 2026 **Series A** 2026 [NFX](https://millionrounds.com/fund/nfx) [2048 Ventures](https://millionrounds.com/fund/2048-ventures) [Insight Partners](https://millionrounds.com/fund/insight-venture-partners) [Alexandria Venture Investments](https://millionrounds.com/fund/alexandria-venture-investments)

## Everyone who backed them

[NFX](https://millionrounds.com/fund/nfx) VC firm **2** rounds

[2048 Ventures](https://millionrounds.com/fund/2048-ventures) VC firm **2** rounds

[Insight Partners](https://millionrounds.com/fund/insight-venture-partners) VC firm **1** round

[Alexandria Venture Investments](https://millionrounds.com/fund/alexandria-venture-investments) VC firm **1** round

Missing a round, or an investor who was in one? [Tell us](https://millionrounds.com/corrections?slug=twostep-therapeutics).

## Quick answers about TwoStep Therapeutics

### Who invested in TwoStep Therapeutics?

TwoStep Therapeutics's investors include NFX, 2048 Ventures, Insight Partners and Alexandria Venture Investments.

### How much has TwoStep Therapeutics raised?

TwoStep Therapeutics has raised $84M across 2 rounds since 2026.

### When did TwoStep Therapeutics last raise?

TwoStep Therapeutics's most recent round was a Series A of $63M in Sept 2026.

### What was TwoStep Therapeutics's biggest round?

Its most recent round, $63M in Sept 2026, is also its largest on record.

### Who backed TwoStep Therapeutics first?

TwoStep Therapeutics's first round on record, in Jan 2026, was backed by NFX, Alexandria Venture Investments and 2048 Ventures.

### What stage is TwoStep Therapeutics at?

TwoStep Therapeutics's latest named round is a Series A, in Sept 2026.

### Where is TwoStep Therapeutics based?

TwoStep Therapeutics is based in the United States.

## Companies like TwoStep Therapeutics

In the same sector, and raised in the last eighteen months.

[**Star Therapeutics** $325M · Oct 2026](https://millionrounds.com/company/star-therapeutics)[**Promaxo** $106M · Oct 2026](https://millionrounds.com/company/promaxo)[**Prolific Machines** $105M · Oct 2026](https://millionrounds.com/company/prolific-machines)[**IMIO** $19M · Oct 2026](https://millionrounds.com/company/imio)[**Clinical Ink** $24M · Oct 2026](https://millionrounds.com/company/clinical-ink)[**Rejuvenate Bio** $13M · Oct 2026](https://millionrounds.com/company/rejuvenate-bio)

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